LncRNA loc339803 acts as CeRNA of miR-30a-5p to promote the migration and invasion of hepatocellular carcinoma cells

Background: Hepatocellular carcinoma (HCC), a most common malignant tumor, has an unfavorable clinical outcome. Emerging evidence demonstrated that long noncoding RNAs (lncRNAs) play important role in the carcinogenesis and progression of HCC. However, significances biological roles lncRNAs HCC remain poorly understood. Methods: The expression levels lncRNA loc339803 tissues cell lines were determined by quantitative real-time polymerase chain reaction (qRT-PCR) assay. cellular sublocalization was fluorescence situ hybridization nuclear cytoplasmic RNA isolation Western blot, CCK-8, Edu, colony formation, migration invasion assays used to investigate vitro. A mouse model for lung metastasis constructed evaluate development vivo. correlations among loc339803, miR-30a-5p SNAIL1 validated qRT-PCR dual- luciferase reporter Results: upregulated lines, positively correlated with tumor size, advanced stage, higher serum AFP level poor prognosis patients. Loc339803 can promote cells vivo Further studies functioned as competing endogenous (ceRNA) directly binding miR-30a-5p, thus up-regulating SNAIL1, target gene miR-30a-5p. Moreover, upregulation blocked enhanced induced overexpression. Conclusions: may be oncogenic associated outcomes. LncRNA might through regulating miR-30a-5p/ axis.